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Inter-cell adhesion molecule 2 (ICAM-2/CD102) elisa kit instruction manual
**Porcine Intercellular Adhesion Molecule 2 (ICAM-2/CD102) ELISA Kit – Instructions for Use**
This ELISA kit is designed for the quantitative determination of Porcine ICAM-2/CD102 in various biological samples. It is intended for research use only and not for diagnostic or therapeutic purposes. The kit is available in two configurations: 48-well and 96-well formats.
**Kit Specifications:**
- **Standard Dilution:** 1.5 mL × 1 vial
- **Enzyme Standard Reagent:** 3 mL × 1 vial (48-well) / 6 mL × 1 vial (96-well)
- **Storage Conditions:** All reagents should be stored at 2–8°C, except for Chromogen B, which should be stored at -20°C.
**Procedure Summary:**
1. Prepare the standard curve by plotting the concentration of the standard on the x-axis and the corresponding OD values on the y-axis.
2. Measure the OD values of your samples and determine their concentrations by referencing the standard curve.
3. Alternatively, calculate the linear regression equation using the standard concentrations and their OD values. Plug in the sample OD value into the equation to find the concentration, then multiply by the dilution factor to obtain the actual sample concentration.
**Kit Components:**
- **Sealing Film:** 2 pieces (for 48-well) / 2 pieces (for 96-well)
- **Standard:** 2700 ng/L, 0.5 mL × 1 vial
- **Enzyme Standard:** 1×48 / 1×96
- **Sample Diluent:** 3 mL × 1 vial (48-well) / 6 mL × 1 vial (96-well)
- **Developer A:** 3 mL × 1 vial (48-well) / 6 mL × 1 vial (96-well)
- **Chromogen B:** 3 mL × 1 vial (48-well) / 6 mL × 1 vial (96-well), store at -20°C
- **Stop Solution:** 3 mL × 1 vial (48-well) / 6 mL × 1 vial (96-well)
- **Concentrated Wash Solution:** 20 mL × 20 times (for 48-well) / 20 mL × 30 times (for 96-well)
**Notes:**
- Ensure all reagents are brought to room temperature before use.
- Avoid contamination during the procedure.
- Follow the manufacturer’s instructions carefully to ensure accurate results.
- Proper disposal of all reagents and waste materials is required.
This kit provides a reliable and sensitive method for measuring ICAM-2 levels in porcine samples, making it a valuable tool for researchers studying cell adhesion and immune responses.