In general, friends who use gas chromatographs for the first time do n’t know how to configure the columns properly. I always hope that the more columns, the better. I blindly purchase many columns or fixatives and supports. Experts recommend some experiences worth considering. Selection of column length: Because the resolution is proportional to the square root of the column length. The longer the column, the higher the resolution. In general: 15m short column is used for rapid separation of simpler samples, and is also suitable for fast component scanning analysis; 30m chromatographic column is the most commonly used column length, most analysis is completed on this length of column; 50m, 60m Or longer columns are used to separate more complex samples. For the choice of caliber: the column diameter directly affects the efficiency, retention characteristics and sample capacity of the column. Small-caliber columns have higher efficiency than larger-caliber columns, but the column capacity is smaller. Equipment Display Rack,Sport Equipment Display Rack,Passenger Cars Tyre Display Rack,Fmcg Display Stand Dongguan Display Leader Co., Ltd , https://www.displayonestop.com
Talking from many years of experience, generally preparing a few columns can basically solve the requirements of various types of gas chromatography analysis, and can preferentially select the fixed liquids as SE-30 (or OV-101), OV-17, PEG-20M , DEGS, FFAP one column each. For packed columns, the support can be selected from white support, red support (including unpickled, pickled, silanized), GDX series (or Porapak series), which can basically solve most of the projects in the work.
0.25mm: It has higher column efficiency and lower column capacity. It is better to separate complex samples.
0.32mm: The column efficiency is slightly lower than 0.25mm, but the column capacity is about 60% higher.
0.53mm: It has a column capacity similar to that of packed columns. It can be used for split injection or splitless injection. When column capacity is the main consideration (such as trace analysis), it is more appropriate to choose a large-diameter capillary column. Experts believe that for ordinary users, a capillary with a length of 30 meters or 50 meters can be selected, and a diameter of 0.32 mm is more convenient.
This article is based on the experience of GC column configuration | Scientific Instrument Online Original Link: http: //