Pollen tube growth is an important example of spontaneous tissue in biological systems. In these spontaneous systems, signals are transmitted through interactive feedback pathways to control and adjust molecular and biochemical parameters to make them most suitable for growth and function. Pollen, as a model material for studying plant cell development, growth and biophysiology, found that the oscillation growth of pollen tubes is related to the influx of Ca2 +, H + and K + cations. In mammalian secretory cells, Cl-channels act as important regulators to regulate secretory activity and maintain cell volume. It has been shown that inositol 3,4,5,6-tetraphosphate negatively regulates Ca2 + activation of Cl-channels. When different inositol phosphates act on pollen tubes, inositol 3,4,5,6-tetraphosphate has the strongest effect. Feijó and other researchers used non-invasive micro-testing techniques to explore the potential relationship between inositol 3,4,5,6-tetraphosphate and Cl-flow. The study found that an oscillating Cl-outflow appeared at the tip of the pollen tube of tobacco and lily, and a continuous inflow along the pollen tube began from 12 μm from the tip. Chloride channel blockers DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid) and 5-nitro-2- (3-phenylpropylamino) -benzoic acid (5-nitro-2- (3- phenylpropylamino) benzoic acid) can completely inhibit the growth of tobacco pollen tubes at 80μM and 20μM, induce cell volume increase, and disrupt the tip Cl-outflow. In addition, the signal encoded by inositol 3,4,5,6-tetraphosphate is not conducive to pollen tube growth, and can induce cell volume to increase, disrupting normal Cl-oscillation outflow. Correlation analysis shows that the period of Cl-outflow coincides with the growth cycle in time, and the Cl-outflow in the growth cycle is related to the movement of vesicles. This study is very important for understanding Cl-kinetics in regulating pollen tube balance and growth networks. Keywords: pollen tube; Chloride flux; inositol 3,4,5,6-tetraphosphate (inositol 3,4,5,6-tetra kisphosphate) Reference: Laura Zonia, et al. The Plant Cell, 2002, 14: 2233-2249 15Ml Glass Perfume Bottles,Blue Glass Perfume Bottle,Small Glass Perfume Bottles,Empty Glass Perfume Bottles Zhejiang Changxing Hanghua Glass Co., Ltd. , https://www.hanghua-glass.com