Frozen Meat Breaker And Grinder
Frozen meat breaker/frozen meat breaker/meat grinder/meat mincer is the first step of high quality products production. They are used for pre-breaking frozen meat blocks stacked on pallets. It includes bone saw series, frozen meat cutting machine, frozen meat flaker,grinder series, etc. Customers will use different machines depending on the processing needs and to form an automatic production line. No need of defrosting of the meat, Helper`s breaker series has been designed to cut off a whole block of meat in meat pieces with a blades system, avoiding second time pollution and keeping meat texture.
Large frozen meat breaker series: Bone saw, Frozen meat cutting machine, frozen meat flaker.
Frozen meat grinder series: JR-120, JR-140, JR-300, chopping and grinding combination system.
Rib Chopping Machine,Frozen Meat Flaker,Frozen Meat Cutter,Meat Mincer,Meat Grinder,Frozen Meat Breaker Helper Machinery Group Co., Ltd. , https://www.ihelpergroup.com
Human cyclic adenosine monophosphate (cAMP) enzyme-linked immunosorbent assay (ELISA) kit instruction manual
This reagent is intended for research use only. It is designed to quantify cyclic adenosine monophosphate (cAMP) in human serum, plasma, and other biological fluids. The assay employs a double-antibody sandwich ELISA method. A microplate is pre-coated with purified anti-cAMP antibodies, which serve as the solid-phase capture reagent. After incubation with the sample, cAMP binds to the immobilized antibody. Subsequently, HRP-conjugated anti-cAMP antibodies are added, forming an immune complex. Following thorough washing, TMB substrate is introduced, leading to a color change from blue to yellow upon acid termination. The intensity of the color correlates directly with the cAMP concentration in the sample. Absorbance is measured at 450 nm using a microplate reader, and concentrations are determined by comparing the sample OD values to a standard curve.
The kit includes: 1 × 48 or 1 × 96 well plates, standard solutions, enzyme-labeled reagents, diluents, wash buffer, and stop solution. All components should be stored at 2–8°C. Proper sample preparation is essential. Serum samples require clotting and centrifugation; plasma should be collected with anticoagulants like EDTA or citrate. Urine and cell culture supernatants need centrifugation as well. Tissue samples must be homogenized in PBS before centrifugation. Avoid repeated freeze-thaw cycles and store samples at -20°C if not tested immediately. Note that NaN3-containing samples may interfere with HRP activity.
Sample processing involves diluting the specimen 5-fold with sample diluent. Standard curves are prepared by serial dilution, typically ranging from 18 to 1.5 nmol/L. Each step must be performed carefully, ensuring accurate pipetting and timing. The sealing film should be used once to prevent contamination. The substrate should be protected from light. Always prepare a standard curve and run duplicates for better accuracy. If a sample’s OD exceeds the highest standard, it should be diluted before testing.
After completing all steps, the absorbance is read at 450 nm. Results can be calculated using linear regression or by plotting the standard curve. The final concentration is obtained by multiplying the measured value by the dilution factor. This kit offers high specificity and sensitivity, with a correlation coefficient (R) of ≥0.92. The detection range is between 1 and 20 nmol/L. The shelf life is 6 months when stored properly. Always follow the manufacturer’s instructions and handle all materials as biohazardous waste. Do not mix reagents from different batches. In case of discrepancy, the English manual takes precedence.