Most aquatic insects are not sensitive to cadmium (Cd2 +) in a short period of time, and the LC50 value of larvae of rock mosquitoes exceeds 25,000 times the maximum concentration standard published by the US Environmental Protection Agency Species Sensitivity Distribution Act. In view of this phenomenon, researchers in Canada studied the flow rate of Cd2 + into and out of tissues, cadmium secretions, and cadmium chelate using the intestine and Malpighian tube of larvae of mosquitoes as materials, and analyzed the mechanism. In this study, the larvae of rock mosquitoes were soaked in a 10 μmol / L Cd2 + solution, and it was found that the Malpighian tube could secrete Cd2 + with complete hemolymph burden within 15 hours. The non-invasive micro-testing technique (SIET) was used to detect along the intestinal cavity of the larvae of rock mosquitoes, and it was found that the intestine was not exactly a barrier for Cd2 + to enter the hemolymph. The key site for Cd2 + to enter the hemolymph is at the front end of the midgut, and the source of Cd2 + at the back end of the midgut is the intestinal lumen. Hemolymph absorption and cadmium chelation in the tissue coexist. The results of this study emphasize that the intestine and Malpighian tube play an important role in the transport and absorption of toxic heavy metal cadmium by the larval larvae. This research is the first to apply cadmium ion selective electrode and non-damage micro-measurement technology to the detection of animal cadmium. It is also the first time to directly detect the transport mechanism of heavy metal cadmium in animal cells and tissues in a physiological sense. Non-damage micro-measurement technology accurately describes the balance mechanism of Cd2 + transport for us, accurately identifies the Cd2 + transport site, and obtains the rate of Cd2 + inflow and outflow in biological samples in real time, providing the most direct evidence for the mechanism of tissue transport of Cd2 + At the same time, it also provides new ideas and models for the study of such problems. Keywords: Cadmium; Cd2 + -selective microelectrodes; Chironomus riparius; Malpighian tubules References: Erin M. Leonard, et al. Aquatic Toxicology, 2009, 92 : 179-186 Changxing Mingwang Rubber & Plastic Technologies Co.,Ltd. , https://www.cxmingwang.com