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Inter-cell adhesion molecule 2 (ICAM-2/CD102) elisa kit instruction manual
**Pig Intercellular Adhesion Molecule 2 (ICAM-2/CD102) ELISA Kit – Instructions for Use**
This ELISA kit is designed for the quantitative determination of Pig ICAM-2/CD102 in various biological samples. It is intended for research use only and not for diagnostic or therapeutic purposes.
**Kit Specifications:**
- **Configuration:** 48-well or 96-well format
- **Standard Dilution:** 1.5 mL × 1 vial
- **Enzyme Standard Reagent:** 3 mL × 1 vial (for 48-well) / 6 mL × 1 vial (for 96-well)
- **Storage Conditions:** All reagents should be stored at 2–8°C, except for Chromogen B, which should be kept at -20°C.
**Procedure Overview:**
To determine the concentration of ICAM-2/CD102 in your samples, follow these steps:
1. **Prepare the Standard Curve:**
- Plot the standard concentrations on the X-axis and corresponding OD values on the Y-axis using a graph paper or software.
- Alternatively, calculate the linear regression equation using the standard concentrations and their respective OD values.
- Substitute the OD value of the sample into the equation to calculate the sample concentration.
- Multiply the result by the dilution factor to obtain the actual concentration of the sample in the original sample.
**Kit Components:**
- **Sealing Film:** 2 pieces (for 48-well), 2 pieces (for 96-well)
- **Standard:** 2700 ng/L, 0.5 mL × 1 vial
- **Enzyme Standard:** 1×48 or 1×96, stored at 2–8°C
- **Sample Diluent:** 3 mL × 1 vial (for 48-well), 6 mL × 1 vial (for 96-well), stored at 2–8°C
- **Developer A:** 3 mL × 1 vial (for 48-well), 6 mL × 1 vial (for 96-well), stored at 2–8°C
- **Chromogen B Solution:** 3 mL × 1 vial (for 48-well), 6 mL × 1 vial (for 96-well), stored at -20°C
- **Stop Solution:** 3 mL × 1 vial (for 48-well), 6 mL × 1 vial (for 96-well), stored at 2–8°C
- **Concentrated Washing Solution:** 20 mL × 20 times or 20 mL × 30 times, stored at 2–8°C
**Notes:**
- Ensure all reagents are brought to room temperature before use.
- Avoid contamination during the procedure.
- Follow the instructions carefully to ensure accurate results.
- Discard any unused reagents after the expiration date.
This kit provides a reliable and sensitive method for measuring ICAM-2/CD102 levels in porcine samples, making it a valuable tool for immunological and inflammatory studies.