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Human S100B protein (S-100B) enzyme-linked immunoassay kit instruction manual
This reagent is for research use only: This kit is used to determine the content of S100B protein (S-100B) in human serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of human S100B protein (S-100B) in the specimen. The microplate was coated with purified human S100B protein (S-100B) antibody to prepare a solid phase antibody, and S100B protein (S-100B) was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP-labeled S100B protein ( The S-100B) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the S100B protein (S-100B) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human S100B protein (S-100B) in the sample was calculated from a standard curve. Kit composition: Kit composition 48-well configuration 96-well configuration Storage instructions 1 part 1 part sealing film 2 pieces (48) 2 pieces (96) Sealed bag 1 1 enzyme label coated plate 1 × 48 1 × 96 2 -8 ° C preservation standard: 1800ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation standard dilution 1.5ml × 1 bottle 1.5ml × 1 bottle 2-8 ° C preservation enzyme standard reagent 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation sample dilution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation developer A solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C preservation Reagent B liquid 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation concentrated washing solution (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle 2-8 ° C preservation