In 1809, the Russian physicist РейÑе first discovered the phenomenon of electrophoresis. He inserted positive and negative electrodes with glass tubes in the wet clay. After applying voltage, he found that the original water layer in the positive glass tube became turbid, that is, the negatively charged clay particles moved to the positive electrode. In 1909 Michaelis first referred to the movement of colloidal ions in an electric field as electrophoresis. He measured the electrophoretic shift and isoelectric point of invertase and catalase in U-shaped tubes with different pH solutions.
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