**Human Znt8A ELISA Kit – For the Quantitative In Vitro Determination of Human Zinc Transporter 8 Autoantibodies in Serum, Plasma, Cerebrospinal Fluid, Tissue Homogenate, and Other Body Fluids** *For Laboratory Research Use Only. Not for Diagnostic or Therapeutic Procedures.* Before using this product, please read the entire package insert carefully. This ELISA (Enzyme-Linked Immunosorbent Assay) is specifically designed for research purposes to quantitatively measure the concentration of Human Zinc Transporter 8 Autoantibodies (Znt8A) in various biological samples. The test works by detecting the presence of Znt8A antibodies in a sample. A set of calibration standards is included with the kit, which allows the user to generate a standard curve based on optical density (OD) readings. By comparing the OD of the unknown samples to this curve, the concentration of Znt8A can be accurately determined. --- **Intended Use:** This Human Znt8A ELISA Kit is intended for laboratory research use only and is not suitable for diagnostic or clinical procedures. It is designed to measure the levels of Znt8A autoantibodies in serum, plasma, cerebrospinal fluid, tissue homogenates, and other body fluids. **Test Principle:** The ELISA method involves binding the target antibody to a microtiter plate coated with specific antigens. After incubation and washing steps, an enzyme-conjugated secondary antibody is added, followed by a chromogenic substrate. The reaction is stopped, and the absorbance is measured at 450 nm. The intensity of the color correlates with the concentration of Znt8A in the sample. --- **Sample Collection and Storage:** - **Serum:** Use a serum separator tube. Allow samples to clot for 2 hours at room temperature or overnight at 4°C. Centrifuge at 2000×g for 20 minutes. Remove serum and assay immediately or store at -20°C. Avoid repeated freeze-thaw cycles. - **Plasma:** Collect using heparin as an anticoagulant. Centrifuge within 30 minutes at 2000×g (2–8°C). Store at -20°C and avoid repeated freezing. - **Tissue Homogenate & Other Fluids:** Centrifuge to remove particulates. Assay immediately or store at -20°C. Avoid repeated freeze-thaw cycles. - *Note: Ensure proper centrifugation and avoid hemolysis or granulation.* --- **Materials Required but Not Supplied:** 1. Incubator at 37°C 2. Microplate reader capable of measuring absorbance at 450 nm 3. Precision pipettes, disposable tips, and absorbent paper 4. Distilled or deionized water --- **Reagents Provided (Stored at 2–8°C):** | Reagent Name | 96 Determinations | 48 Determinations | |--------------|-------------------|-------------------| | MicroELISA Stripplate | 12 × 8 strips | 12 × 4 strips | | Standard (6 vials) | 0.5 ml/vial | 0.5 ml/vial | | Sample Diluent | 6.0 ml | 3.0 ml | | HRP-Conjugate Reagent | 10.0 ml | 5.0 ml | | 20X Wash Solution | 25 ml | 15 ml | | Chromogen Solution A | 6.0 ml | 3.0 ml | | Chromogen Solution B | 6.0 ml | 3.0 ml | | Stop Solution | 6.0 ml | 3.0 ml | | Closure Plate Membrane | 2 | 2 | | User Manual | 1 | 1 | | Sealed Bags | 1 | 1 | *Standard concentrations: 200, 100, 50, 25, 12.5, 6.25 pg/mL.* *If sample values exceed the highest standard, dilute with Sample Diluent and repeat the assay.* --- **Precautions:** - Do not mix reagents from different kit lots. - All reagents must reach room temperature before use. - Do not use expired reagents. - Use only deionized or distilled water for dilution. - Store unused strips in their sealed pouch with desiccant. - Wear gloves during handling of biological samples. - Dispose of all waste properly after inactivation (minimum 30 minutes). - Avoid contamination; use fresh pipette tips for each transfer. - Handle all samples as potentially infectious. --- **Reagent Preparation and Storage:** - **Wash Solution (1X):** Dilute 1 volume of 20X Wash Solution with 19 volumes of distilled water. Store at 2–8°C for up to 1 month. --- **Assay Procedure:** 1. Prepare all reagents before starting. Add standards and samples in duplicate. 2. Add 50 μL of standard or sample to each well (blank well receives no addition). 3. Add 100 μL of HRP-Conjugate Reagent to all wells except blank. Cover and incubate for 60 minutes at 37°C. 4. Wash the plate 4 times manually or automatically. 5. Add 50 μL of Chromogen A and 50 μL of Chromogen B. Incubate for 15 minutes at 37°C, protected from light. 6. Add 50 μL of Stop Solution. Color changes from blue to yellow. If uneven, gently tap the plate. 7. Read OD at 450 nm. Generate a standard curve by plotting OD vs. concentration. --- **Interpretation of Results:** - Calculate the mean OD for each standard and sample. Subtract the blank OD value. - Plot the standard curve and determine sample concentrations accordingly. - Intra-assay and inter-assay CV% are <15%. - Sensitivity: <1.0 pg/mL. - Assay range: 6.25 pg/mL – 200 pg/mL. - No cross-reactivity or interference observed. **Storage:** - Store at 2–8°C (for frequent use) or -20°C (long-term). - Shelf life: 6 months when stored properly. **Important Notes:** - Always follow good laboratory practices. - This kit is not intended for diagnostic use. - Consult the user manual for full details and troubleshooting. **Please read all instructions thoroughly before performing the assay.**

Kid's Furniture Sets

Simple design :Kid Furniture Sets Homebase comes with 1 chair, made from faux wood, and the modern two-color design of the table in modern shades of gray and white ensures it looks just as cool in a playroom or living room.
Kid Furniture Kit: Movable table with storage features an extended two-sided game table with a reversible game board compatible with most major blocks. On the other side is a smooth surface perfect for model trains, art projects, tea parties, snacks, dollhouses and other toys. You can use Kid Furniture Sets Lowes as kids' activity tables, normal tables, play tables.
Large storage space: Under the art surface, an easily accessible storage compartment for building bricks, paintbrushes, toys, and other items. This children's table and chair set is compatible with Lego bricks and will add a nice touch to any room. Tables with building panels: The building panels above Quality Kid Furniture are compatible with most major building bricks. This wooden children's dining table is the perfect gift for children aged 6-12; This activity play table with storage size is suitable for toddlers, children, older children over 3 years old.

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