N-phenyl-8-naphthylamine-1-sulfonic acid / 8-anilino-1-naphthalenesulfonic acid / 8-anilino-1-naphthalenesulfonic acid

Cas No. 82-76-8
Molecular formula C16H13SO3; C6H5NHC10H6SO3H
Molecular weight 299.34
8-Anilino-1-naphthalenesulfonic acid
Its sodium salt is light green to gray needle-like or flaky crystal, insoluble in water, and its ammonium salt hydrate is soluble in ethanol.
Melting point: 215-217 ° C
Purity> 95.0% (neutralization titration)

Product use dye intermediates, used to produce weak acid black BR, weak acid dark blue 5R, sulfur dyes and azo dyes.
1,8-ANS is a fluorescent dye with high affinity for the hydrophobic surface of proteins. After 1,8-ANS undergoes blue shift and the fluorescence intensity increases significantly, it binds to the protein surface of low-polarity areas1,2. These properties make 1,8-ANS very suitable for the determination of affinity for hydrophobic ligands and their corresponding binding proteins, such as fatty acid binding proteins (FABPs) with hydrophobic free fatty acids.

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