This kit can only be used for scientific research, not for medical diagnosis. Quantitative Test Kit (ELISA) for Rat Type â…£ Collagen (Col â…£) user's Guide ã€Kit name】 Quantitative Test Kit (ELISA) for Rat Type â…£ Collagen (Col â…£) ã€Use of the kit】 Quantitatively detect the content of type â…£ collagen (Col â…£) in rat serum, plasma and related liquid samples. ã€Detection principle】 This kit uses double-antibody two-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the standard and the test sample to the pre-coated rat collagen type IV (Col â…£) monoclonal antibody transparent enzyme label coated plate. After incubation for a sufficient time, wash to remove unbound components, and then add the enzyme label After incubation for a sufficient period of time, wash to remove unbound components. Substrates A and B were added in sequence, and the substrate (TMB) was converted into a blue product catalyzed by horseradish peroxidase (HRP), which turned yellow under the action of an acid. The concentration of collagen (Col â…£) was positively correlated. The OD value was measured at a wavelength of 450 nm. Based on the OD value of the standard and the sample, the content of rat type IV collagen (Col â…£) in the sample was calculated. Ionic Hair Dryer,Hooded Hair Dryer,Small Hair Dryer,Rotating Hair Brush Dryer Ningbo Meirou Electric Appliance Co.,Ltd. , https://www.mrhairdryer.com