August 09, 2024

Problems that should be paid attention to when using flow cytometry to count cells

After making tissue homogenate or cell suspension and staining with fluorescent dye, you can use flow cytometry to count the number of cells with different DNA content or multiples. For example, in testicular tissue, sperm cells are haploid cells, spermatogonium cells, Sertoli cells, Leydig cells, and other non-spermatogenic cells are diploid cells, primary spermatocytes, and spermatogonium cells and non-squamous cells in the dividing stage Spermatogenic cells are tetraploid cells. Therefore, using flow cytometry to determine the changes in DNA ploidy of spermatogenic cells at all levels of testicular tissue can indirectly determine the trend of the relative proportion of different spermatogenic cell numbers. However, you must be cautious when using these results to draw conclusions. For example, a decrease in the percentage of haploid cells may indicate a decrease in the number of sperm cells, but the following three conditions may also cause a decrease in the percentage:

First, the number of haploid cells remains unchanged, and the number of other ploidy cells increases;

Second, the number of haploid cells increased, and the number of other ploidy cells also increased and increased relatively more;

The third is that the number of haploid cells is reduced, and the number of other ploidy cells is also reduced, but the reduction is relatively small.

Similarly, the same percentage may indicate that spermatogenesis has not been affected, but this result may also occur in the following two situations: haploid cells and other ploidy cells increase proportionally; haploid cells and other ploidy cells The cells are reduced proportionally. Therefore, regardless of the accurate identification of cells and the problem of cell crushing during tissue homogenization, flow cytometry estimates that the number of cells has at least such a defect: generally it can only reflect the relative number ratio of cells, and cannot determine the measured cells in a certain Whether the total number of areas of interest has really increased, decreased or remained the same.

Display Storage Cabinets

Priceless memories from that holiday trip, your grandmothers old clock or just a fine piece of ceramic. Most of us collect things that we love to see every day. A storage cabinet is a perfect way to keep our rarities visible, yet away from dust and smudgy fingers.Need a little extra storage space or just a great looking display cabinet? It is a fabulous choice to display your daily essentials, favorite collection or priceless knickknacks, even use as a very cool pantry in the kitchen. The Washed Linen finish with a darker pine interior give it a fresh, casual, stylish look. The doors are fitted with metal wire that can lend itself to a rustic, farmhouse feel, or just add to the interest of your own design.

Display Storage Cabinets,Shelving Display Units,Display Shelf Cabinet,Display Unit With Drawers

HOPE IKEA , https://www.skyfurnishing.com

The application of thermal analysis in the pharmaceutical and food industry-the third round of the prize survey activity is looking forward to your joining!

It is said that this smart packaging box can reduce the company's packaging costs by 90%, Huang Hao also voted!