Selection of ELISA kit

ELISA kit selection should pay attention to the following points:

1. The Ministry of Health stipulates that hepatitis B, hepatitis C, AIDS, syphilis reagents and blood group reagents must be qualified by the Ministry of Health Biological Products Inspection Institute and have anti-counterfeit labels. Make a comprehensive evaluation of safety and economy. Refers to the reagent's ability to correctly detect non-existent substances (no false positives), depending on the purity and specificity of the coating antigen (body) and the labeled antigen (body).

2. Specificity: Sensitivity: including 1) the ability of the reagent to detect the minimum amount of the tested substance; 2) the ability of the reagent to positively detect the population or a large number of samples (the fewer false negatives the better), depending on The comprehensiveness of the object.

3. Precision: ELISA reagent generally refers to the CV within the batch, and its value should be less than 15%; the quantitative reagent should also examine the linear range

4. Convenience: Refers to the previous question that does not affect the first three indicators of the reagents, the fewer the experiment and the measurement steps, the better, the result judgment in the qualitative test is simple and clear,

5. Stability: The time that the reagent can be stored under the specified conditions. Generally, a destructive test is used to store the reagent at 37 ℃, and its sensitivity, specificity and precision are regularly measured until its quality index begins to decline. Usually It is believed that each stable day at 37 ° C is equivalent to one and a half months at 4-10 ° C.

6. Economical efficiency: Reagents reduce costs through large-scale production or technological progress under the same quality conditions and the market price is relatively reasonable.

7. Safety: Refers to reagents that are safe and harmless to operators and the environment.

8. Reagent evaluation requires an authoritative serum assessment panel (Panel) for testing. It is not easy for general laboratories to obtain from the biopsy or clinical inspection center. It is also troublesome to enter the reagent evaluation every time. Reagents can be selected through indirect information.

9. Refer to the evaluation results of reagents in the inter-room quality evaluation evaluation report, which is more objective and fair, because the statistics are from the participating hospitals, reflecting the use of reagents in a certain area;

10. Determine the quality of the reagent by asking the composition of the reagent coating, such as the source of the raw material (genetic engineering or synthetic polypeptide), the combination of fragments (proportionally mixed or chemically synthesized), and the length of the fragments;

11. According to the batch verification report of the reagent bioassay institute, understand its quality level, and select the reagent with high sensitivity or high specificity according to the quality plan;

12. According to the reagent evaluation results released by authoritative departments, understand the quality of reagents on the market.

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PERCELL PET is established in 1978 with offices located in Taipei, Taiwan and Guangdong, China. Currently, we partners with more than 49 distributors around the world and carries thousands of quality pet supplies for dogs, cats, birds, fresh and salt water fishes, reptiles and small animals such as rabbits and ferrets, etc from both Taiwan and China.

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