Principle of ELISA Kit for Human Immunoglobulin G Fc Segment Receptor â… (FcγRâ… / CD64) 1. The level of human immunoglobulin G Fc-segment receptor â… (FcγRâ… / CD64) in specimens was determined by double antibody sandwich method. 2. First, coat the microplate with purified human immunoglobulin G Fc segment receptor I (FcγRI / CD64) antibody to prepare a solid-phase antibody. 3. Then add immunoglobulin G Fc-segment receptor I (FcγRâ… / CD64) to the microwells coated with mAb in sequence, and then combine with HRP-labeled immunoglobulin G Fc-segment receptor I (FcγRâ… / CD64) antibody An antibody-antigen-enzyme-labeled antibody complex is formed, and after thorough washing, a substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. 4. The color depth is positively correlated with the immunoglobulin immunoglobulin G Fc segment receptor â… (FcγRâ… / CD64) in the sample. 5. Finally, the absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the concentration of human immunoglobulin G Fc segment receptor â… (FcγRâ… / CD64) in the sample is calculated from the standard curve. Purpose of use: To determine the content of immunoglobulin G Fc segment receptor â… (FcγRâ… / CD64) in human serum, plasma and related liquid samples. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm) Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins. 2. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully. If a precipitate forms, it should be centrifuged again. safety: 4 Keep Elisa kit away from children Bring your own items: 1. 37 ℃ incubator 2. Standard specification microplate reader 3. Precision pipette and disposable tip 4. Distilled water 5. Disposable test tube 6. Absorbent paper Eyeshadow powder Eye shadow Stick,Waterproof Eye Shadow Stick,Liquid Waterproof Eyeshadow,Pigment Shimmer Eyeshadow Stick Henan Daxin Cosmetics Co., Ltd. , https://www.dx-cosmetics.com
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
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